Effect of Exogenous NO on the Plant Growth and Antioxidant System of Eggplant Seedlings under High Temperature Stress
1 Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China
2 Horticultural Research Institute, Zhumadian Academy of Agricultural Sciences, Zhumadian, 463000, China
International Journal of Horticulture, 2022, Vol. 12, No. 5 doi: 10.5376/ijh.2022.12.0005
Received: 08 Dec., 2022 Accepted: 16 Dec., 2022 Published: 26 Dec., 2022
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This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Wu X.X., Wang Y., Jiang J., Shen H.B., Tian S.B., and Zha D.S., 2022, Effect of exogenous NO on the plant growth and antioxidant system of eggplant seedlings under high temperature stress, International Journal of Horticulture, 12(5): 1-13 (doi: 10.5376/ijh.2022.12.0005)
Effects of 0.05~5mmol/L sodium nitroprusside (SNP, nitricoxidedonor) on the growth, antioxidant enzyme activities and reactive oxygen metabolism in leaves and growth of eggplant seedlings with spraying exogenous were investigated, using eggplant variety of ‘Tewangd’ under high-temperature stress ((43±1)°C/(38±1)°C(day/night)). The results showed that: (1) The plant growth was significantly inhibited as well as the chlorophyll content was significantly decreased under high temperature stress. Different concentrations of SNP treatments could enhance the seedlings growth and increase the chlorophyll content, and the most effective treatment is 0.1 mmol/L SNP. (2) The content of malondialdehyde (MDA), superoxide anion (O2•¯) producing rate and hydrogen peroxide (H2O2) in eggplant seedlings under high temperature stress were significantly decreased after 0.1 mmol/L SNP treatment. Meanwhile, the content of permeable substances (proline, soluble protein, soluble sugar) increased significantly in eggplant seedlings. (3) The superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) and ascorbic acid peroxidase (APX), dehydrogenation ascorbic acid reductase (DHAR), single dehydrogenation ascorbic acid reductase (MDHAR), glutathione reductase (GR) activities of antioxidant enzymes and antioxidants such as ascorbic acid (AsA) and glutathione (GSH) contents of eggplant seedlings under high temperature stress, after dealing with the 0.1 mmol/L SNP, were significantly increased, however, the AsA/DHA, GSH/GSSG ratio were reduced. The addition of NO scavenger hemoglobin partially or completely reversed the above effects. In this study, the physiological effects of exogenous NO on the resistance of eggplant to high temperature stress were investigated in order to provide theoretical basis for the application of NO in facility cultivation. This study shows that the activity of antioxidant enzymes were further improved, the osmotic regulation substances contents were increased, the stability of cell membrane system was maintained and the inhibition of seedling growth was repaired under high temperature stress with the exogenous 0.1 mmol/L SNP treatment. This study may provide the theory basis for the exogenous NO application in facility culture.
Exogenous NO; Eggplant; High temperature stress; Seedling growth; Antioxidation