Cloning and Expression Pattern Analysis of Flowering Related Gene ApVIN3 from Baizilian (Agapanthus praecox ssp. orientalis)
1 College of Modern Agriculture, Jiaxing Vocational and Techinical College, Jiaxing, 314036, China
2 College of Landscape Engineering, Suzhou, Suzhou Polytechinic Institute of Agriculture, 215008, China
3 Garden Department, Shanghai Vocational Technical College of Agriculture and Forestry, Shanghai, 201600, China
International Journal of Horticulture, 2023, Vol. 13, No. 3 doi: 10.5376/ijh.2023.13.0003
Received: 21 Mar., 2023 Accepted: 30 Jun., 2023 Published: 31 Jul., 2023
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This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Shi Y.B., Wang C.Z., and Zhang Y., 2023, Cloning and expression pattern analysis of flowering related gene ApVIN3 from Baizilian (Agapanthus praecox ssp. orientalis), International Journal of Horticulture, 13(3): 1-8 (doi: 10.5376/ijh.2023.13.0003)
A core fragment highly homologous with VERNALIZATION INSENSITIVE 3(VIN3) was obtained on the basis of preliminary Agapanthus praecox ssp. orientalis transcriptome sequencing results. The full length sequence of cDNA, VIN3 gene of A. praecox was gained by the RACE method, which was named ApVIN3 with the accession number of KJ472789. The results of sequence analysis showed that the ApVIN3 gene length of cDNA was 1 943 bp, which contained an open reading frame of 1 680 bp, 559 encoding amino acids, untranslated region (UTR) 5’ and 3’ with the length of 88 bp and 175 bp, respectively. ApVIN3 encoding is a PHD-finger protein with a conserved sequence structure of C4HC3 (cys4-His-cys3), which is highly similar to the VIN3 protein of Brachypodium distachyum (L.) Beauv (XP_003573553.1). Quantitative Real-time PCR show: The regulation of gene expression in quantity by temperature in Agapanthus africanus Hoffmgg. shoot apex, with prolonging the time of low temperature rising.This gene may play an important role in the process of brucella induction, and its function and regulation mechanism can be studied deeply.
Agapanthus praecox ssp. Orientalis; ApVIN3; Clone; Gene expression
International Journal of Horticulture
• Volume 13