Yuannan Hu¹ , Zhizhong Zhao² , Dan Wu² , Jing Qiu¹ , Wei Liu¹ , Guangying Chen¹ , Wenying He¹

1 Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education, College of Chemical and Chemical Engineering, Hainan Normal University, Haikou, 571158, China
2 College of Geography and Tourism, Hainan Normal University, Haikou, 571158, China
Author    Correspondence author
International Journal of Horticulture, 2023, Vol. 13, No. 1   doi: 10.5376/ijh.2023.13.0001
Received: 10 Feb., 2023    Accepted: 17 Feb., 2023    Published: 28 Feb., 2023

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Hu Y.N., Zhao Z.Z., Wu D., Qiu J., Liu W., Chen G.Y., and He W.Y., 2023, The comparison between the yellow and pink flowers harvested from Hainan Lantana camara L. on the vegetable proteins and metallic elements, International Journal of Horticulture, 13(1): 1-13 (doi: 10.5376/ijh.2023.13.0001)

In this study, the yellow and pink flowers of Lantana camara L. harvested from Hainan were used as the experimental samples, two-dimensional electrophoresis (2-DE) based on BPP + phenol extraction was used to separate and extract the proteins from the yellow flower and pink flowers harvested from Hainan Lantana camara L. under the optimized conditions. The gel images of the well-expressed protein were obtained, which were analyzed by using Image Master5.0 software. Then the corresponding enzymatic hydrolysis products were identified by MALDI-TOF-MS. 23 kinds of the differentially expressed proteins from the yellow flowers and 25 kinds from pink flowers of Lantana camara L. were determined by means of bioinformatics. There were 9 kinds of common proteins, 6 kinds of peculiarly expressed proteins in yellow flower and 7 kinds of peculiarly expressed proteins in pink flower, respectively. In addition, a microwave digestion inductively coupled plasma mass spectrometry (ICP-MS) was performed to analyze 25 kinds of metal elements and their contents including K, Ca, Mg, Na, Al, Fe, Mn, Cu and Zn etc. The result indicated that the contents of K, Al, Mg and Fe are higher. Based on the above experimental results, it is the first time to measure the types and contents of vegetable proteins and metal elements contained in the flowers of Lantana camara L.. It is possible for further explanations on its pharmacological activity as a botanical drug from the point of proteins and metal elements, which provides a theoretical guidance for the further development and utilization of Hainan characteristic economic crops.

Lantana camara L.; Differentially expressed proteins; Metallic element; ICP-MS