Research Report

Effect of Growth Regulators on Rooting of Carnation (Dianthus caryophyllus) Tissue Culture Seedlings  

Jingjie Lu1* , Xijile Bao2* , Mengdi Zhang1 , Yongxiu Zhao1 , Qiqige Alatan1
1 School of Life Sciences, Inner Mongolia University, Hohhot, 010070, China
2 College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China
*These authors contributed equally to this work
Author    Correspondence author
International Journal of Horticulture, 2021, Vol. 11, No. 1   doi: 10.5376/ijh.2021.11.0001
Received: 19 Apr., 2021    Accepted: 23 Apr., 2021    Published: 07 May, 2021
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This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Lu J.J., Bao X.J.L., Zhang M.D., Zhao Y.X., and Alatan Q.Q.G., 2021, Effect of growth regulators on rooting of carnation (Dianthus caryophyllus) tissue culture seedlings, International Journal of Horticulture, 11(1): 1-6 (doi: 10.5376/ijh.2021.11.0001)


In order to understand the effects of different growth regulators and their concentrations on the rooting culture of carnation tissue culture seedlings, the optimal growth regulators and their concentrations were explored by adding different concentrations of IAA and IBA to the basic culture medium for tissue culture experiments and observing the rooting and growth of carnation tissue culture seedlings. The results showed that the sterilization effect of 10% sodium hypochlorite for 4 min was the best; the root length and number of roots in MS medium were significantly higher than that in 1/2 MS and 2 MS medium, and the roots were faster, the rooting rate was high, and the plant growth was good; IAA and IBA of 1.0 mg/L were all conducive to the root elongation, and significantly increased the number of roots, the rest of the concentration promoting effect was not significant, and the high concentration would inhibit the root growth. The growth of the plants treated with IBA was better, and the best was 1.0 mg/L IBA, which was most conducive to the rooting culture of carnation. The above results provide further theoretical basis for in vitro rapid propagation and industrial production of carnation cut flowers.

Carnation (Dianthus caryophyllus); Growth regulator; Rooting culture
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International Journal of Horticulture
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