Siddra Ijaz , Irfan Arshad , Nazish Jahan , Imran ul Haq

1 Centre of Agricultural Biochemistry and Biotechnology/US-Pak Center for Advanced Studies in Agriculture and Food Security, University of Agriculture Faisalabad, Pakistan
2 Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture Faisalabad, Pakistan
3 Department of chemistry, University of Agriculture Faisalabad, Pakistan
4 Department of Plant Pathology, University of Agriculture Faisalabad, Pakistan
Author    Correspondence author
International Journal of Horticulture, 2017, Vol. 7, No. 18   doi: 10.5376/ijh.2017.07.0018
Received: 09 Jun., 2017    Accepted: 01 Jul., 2017    Published: 21 Jul., 2017

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Ijaz S., Arshad I., Jahan N., and Imran-ul-Haq, 2017, Assessing the effect of naphthalene acetic acid and 6-Benzylaminopurine on in vitro micropropagation of potato (Solanum tuberosum L.) and estimation of secondary metabolites in in vitro micropropagated shoots, International Journal of Horticulture, 7(18): 146-153 (doi: 10.5376/ijh.2017.07.0018)

The effect of Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP) was investigated on in vitro micropropagation of potato (Solanum tuberosum L.) and secondary metabolites of in vitro micropropagated shoots were also estimated. Eight micropropagation media containing different level of NAA and BAP were studied and the effect of NAA and BAP on in vitro micropropagated shoots was assessed. However, in this research study, medium MPm 7 having 2.75 mg/l BAP was proved to be best for giving maximum number of shoots per explant in both genotypes to be studied. The genotype, PRI-Red yielded maximum number of shoots on this medium thus these in vitro micropropagated shoots were subjected to phytochemical analyses and were compared with shoots of intact plant (field grown) for secondary metabolites viz., alkaloids, flavonide and total polyphenolic contents. In phytochemical analyses, no alkaloids were not detected in both in vitro micropropagated shoots and shoots of intact plant while both plants showed positive test for presence of polyphenolics. Shoots of intact plant contained higher amount of total polyphenolics (3.7 GAE mg/g fresh wt.) as compared to in vitro micropropagated shoots (2.8 GAE mg/g fresh wt.). However, slightly higher amount of flavonide were detected in in vitro micropropagated shoots (1.8 mg/g) than shoots of intact plant (1.6 mg/g).

Potato; Polyphenoles; Micropropagation; 6-Benzylaminopurine; Naphthalene acetic acid