Cloning and Sequence Analysis of SVP and AGL24 Genes in Chrysanthemum
College of Architecture, Inner Mongolia University of Science and Technology, Baotou, 014010
International Journal of Horticulture, 2020, Vol. 10, No. 2
Received: 17 Jun., 2020 Accepted: 19 Jun., 2020 Published: 19 Jun., 2020
© 2020 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The SVP (SHORT VEGETATIVE PHASE)/AGL24 (AGAMOUS-LIKE 24) gene is a member of the MADS-box family and plays an important role in the transformation of plants from vegetative to reproductive growth. In order to understand the mechanism of flowering transformation in Chrysanthemum morifolium, two SVP/AGL24 homologous genes named CmSVP1 and CmAGL24 were cloned from C. morifolium 'Jinbudiao' using RT-PCR. Biological information indicated that the coding regions of CmSVP1 and CmAGL24 were 678 bp and 468 bp in length, encoding 225 and 154 amino acids respectively. The subcellular localization predicition of CmSVP1 and CmAGL24 proteins were both in the nucleus. They were hydrophilic proteins, and the α-helix was the largest structural component in the protein secondary structure. The analysis of transmembrane regions suggested that these proteins may not have transmembrane regions. Further proteins homology comparisons showed that the CmSVP1 and TcSVP were clustered into one group, while CmAGL24 was recently related to AaAGL24. This study provides a theoretical basis for analyzing the molecular mechanism of chrysanthemum flowering regulation and improving flowering by molecular means.
Chrysanthemum morifolium; SVP/AGL24 gene; Clone; Biological information analysis
International Journal of Horticulture
• Volume 10