Research Report

Cloning and Sequence Analysis of SVP and AGL24 Genes in Chrysanthemum  

Yaohui Gao , Bin Ma , Guangpu Wei , Fengjie Xiao , Xiaomin Zhang
College of Architecture, Inner Mongolia University of Science and Technology, Baotou, 014010
Author    Correspondence author
International Journal of Horticulture, 2020, Vol. 10, No. 2   
Received: 17 Jun., 2020    Accepted: 19 Jun., 2020    Published: 19 Jun., 2020
© 2020 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract

The SVP (SHORT VEGETATIVE PHASE)/AGL24 (AGAMOUS-LIKE 24) gene is a member of the MADS-box family and plays an important role in the transformation of plants from vegetative to reproductive growth. In order to understand the mechanism of flowering transformation in Chrysanthemum morifolium, two SVP/AGL24 homologous genes named CmSVP1 and CmAGL24 were cloned from C. morifolium 'Jinbudiao' using RT-PCR. Biological information indicated that the coding regions of CmSVP1 and CmAGL24 were 678 bp and 468 bp in length, encoding 225 and 154 amino acids respectively. The subcellular localization predicition of CmSVP1 and CmAGL24 proteins were both in the nucleus. They were hydrophilic proteins, and the α-helix was the largest structural component in the protein secondary structure. The analysis of transmembrane regions suggested that these proteins may not have transmembrane regions. Further proteins homology comparisons showed that the CmSVP1 and TcSVP were clustered into one group, while CmAGL24 was recently related to AaAGL24. This study provides a theoretical basis for analyzing the molecular mechanism of chrysanthemum flowering regulation and improving flowering by molecular means.

Keywords
Chrysanthemum morifolium; SVP/AGL24 gene; Clone; Biological information analysis
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International Journal of Horticulture
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. Yaohui Gao
. Bin Ma
. Guangpu Wei
. Fengjie Xiao
. Xiaomin Zhang
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. Chrysanthemum morifolium
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