Weidong Zhu

Zhejiang Shouxiangu Pharmaceutical Co., Ltd., Jinhua, 321200, Zhejiang, China
Author    Correspondence author
Medicinal Plant Research, 2024, Vol. 14, No. 4   doi: 10.5376/mpr.2024.14.0019
Received: 05 Jul., 2024    Accepted: 09 Aug., 2024    Published: 25 Aug., 2024

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Zhu W.D., 2024, Regulation of secondary metabolite pathways in Ganoderma lucidum under environmental stress, Medicinal Plant Research, 14(4): 223-233 (doi: 10.5376/mpr.2024.14.0019)

This study explores the regulatory mechanisms of secondary metabolic pathways, particularly the biosynthesis of ganoderic acid (GA), in Ganoderma lucidum under various environmental stress conditions. The research identified several key environmental factors influencing GA biosynthesis, including water stress, heat stress, and nitrogen limitation. Water stress was found to increase intracellular reactive oxygen species (ROS) levels and GA content, mediated by the interaction between aquaporin (GlAQP) and NADPH oxidase (NOX). Heat stress was shown to inhibit mycelium growth and induce GA biosynthesis through increased cytosolic Ca²⁺ concentration and membrane fluidity. Additionally, the mitochondrial pyruvate carrier (MPC) regulated by GCN4 was found to play a crucial role in GA biosynthesis under nitrogen limitation. Phospholipase D (PLD) and phosphatidic acid (PA) were also implicated in heat stress-induced GA biosynthesis. The findings highlight the complex regulatory mechanisms of GA biosynthesis in G. lucidum under environmental stress, involving multiple signaling pathways and metabolic adjustments. These insights provide a foundation for further research on fungal secondary metabolism and potential biotechnological applications.

Ganoderma lucidum; Ganoderic acid; Environmental stress; Secondary metabolism; Reactive oxygen species; Cytosolic Ca²⁺; Mitochondrial pyruvate carrier; Phospholipase D