Chuchu Liu

Institute of Life Science, Jiyang College of Zhejiang A&F University, Zhuji, 311800, China
Author    Correspondence author
Medicinal Plant Research, 2023, Vol. 13, No. 3   doi: 10.5376/mpr.2023.13.0003
Received: 31 Aug., 2023    Accepted: 12 Oct., 2023    Published: 20 Oct., 2023

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Liu C.C., 2023, Targeted editing of the ginsenoside biosynthesis pathway in ginseng using CRISPR/Cas9 technology and its implications, Medicinal Plant Research, 13(3): 1-12 (doi: 10.5376/mpr.2023.13.0003)

CRISPR/Cas9 technology, as a revolutionary gene editing tool, has garnered widespread attention in the field of plant biology. This review focuses on ginseng (Panax ginseng) and explores the application of CRISPR/Cas9 technology in targeted editing of the ginsenoside biosynthesis pathway, as well as its potential impact on medicinal value. Panax ginseng, a significant traditional medicinal herb, contains bioactive compounds known as ginsenoside, which possess various medicinal properties such as immunomodulation, anti-fatigue, and anti-cancer effects. To enhance ginsenoside production, the precise regulation of its biosynthesis pathway through CRISPR/Cas9 gene editing has emerged as an effective strategy. This review elaborates on the ginsenoside biosynthesis pathway, the advancements in CRISPR/Cas9 technology, and the latest research progress in manipulating the ginsenoside biosynthesis pathway using this technique. Through this review, we delve into the application of CRISPR/Cas9 technology in the ginsenoside biosynthesis pathway of ginseng, providing a theoretical basis and technical reference for further exploring the medicinal potential of ginseng.

CRISPR/Cas9 technology; Ginsenoside; Biosynthesis pathway; Site-specific gene editing; Medicinal value