Review and Prospect
Targeted Editing of the Ginsenoside Biosynthesis Pathway in Ginseng Using CRISPR/Cas9 Technology and Its Implications
Institute of Life Science, Jiyang College of Zhejiang A&F University, Zhuji, 311800, China
Medicinal Plant Research, 2023, Vol. 13, No. 3 doi: 10.5376/mpr.2023.13.0003
Received: 31 Aug., 2023 Accepted: 12 Oct., 2023 Published: 20 Oct., 2023
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Preferred citation for this article:
Liu C.C., 2023, Targeted editing of the ginsenoside biosynthesis pathway in ginseng using CRISPR/Cas9 technology and its implications, Medicinal Plant Research, 13(3): 1-12 (doi: 10.5376/mpr.2023.13.0003)
CRISPR/Cas9 technology, as a revolutionary gene editing tool, has garnered widespread attention in the field of plant biology. This review focuses on ginseng (Panax ginseng) and explores the application of CRISPR/Cas9 technology in targeted editing of the ginsenoside biosynthesis pathway, as well as its potential impact on medicinal value. Panax ginseng, a significant traditional medicinal herb, contains bioactive compounds known as ginsenoside, which possess various medicinal properties such as immunomodulation, anti-fatigue, and anti-cancer effects. To enhance ginsenoside production, the precise regulation of its biosynthesis pathway through CRISPR/Cas9 gene editing has emerged as an effective strategy. This review elaborates on the ginsenoside biosynthesis pathway, the advancements in CRISPR/Cas9 technology, and the latest research progress in manipulating the ginsenoside biosynthesis pathway using this technique. Through this review, we delve into the application of CRISPR/Cas9 technology in the ginsenoside biosynthesis pathway of ginseng, providing a theoretical basis and technical reference for further exploring the medicinal potential of ginseng.
CRISPR/Cas9 technology; Ginsenoside; Biosynthesis pathway; Site-specific gene editing; Medicinal value