Ying Zhang² , Xiaomeng Luo³ , Xingju Luo² , Shuili Zhang¹ , Hong Wang¹ , Chunchun Zhang¹ , Huiyan Fan¹

1 College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, China
2 College of Binjiang, Zhejiang Chinese Medical University, Hangzhou, 310053, China
3 Taizhou Traditional Chinese Medicine Hospital, Taizhou, 318020, China
Author    Correspondence author
Medicinal Plant Research, 2023, Vol. 13, No. 1   doi: 10.5376/mpr.2023.13.0001
Received: 17 Apr., 2023    Accepted: 26 Apr., 2023    Published: 10 May, 2023

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Zhang Y., Luo X.M., Luo X.J., Zhang S.L., Wang H., Zhang C.C., and Fan H.Y., 2023, Comparison and improvement of total rna extraction methods from different tissues of Polygonatum cyrtonema Hua., Medicinal Plant Research, 13(1): 1-9 (doi: 10.5376/mpr.2023.13.0001)

Extraction and isolation of high-quality RNA from Polygonatum cyrtonema Hua. is the basis of research on gene expression, regulation and genetic engineering. For screening the best method of total RNA extraction from different tissues of P. cyrtonema, total RNA was extracted from rhizomes, stems, leaves and flowers of P. cyrtonema by six methods that were Trizol method, CTAB-isopropanol method, RNA pure plant kit CTAB-LiCl method, hot phenol method and improved hot phenol method respectively. The concentration and quality of RNA were analyzed using Subordinate function method. The results showed that the improved hot phenol method was the most ideal for RNA extraction among the six methods. The RNA bands of different tissues of P. cyrtonema were complete and clear, the OD₂₆₀/OD₂₈₀ and OD₂₆₀/OD₂₃₀ values were between 1.8 and 2.1, the extraction concentration values were between 77.16 and 185.72 µg/g. This study provide a reference for extracting high-quality total RNA from P. cyrtonema.

Polygonatum cyrtonema Hua.; RNA extraction; Improved hot phenol method; Subordinate function method