Research Report

Effects of Plant Growth Regulators for Seed Embryos Development of Cremastra appendiculata  

Li Tian , Yanyan Gao , Ningxian Yang , Sijing Peng , Mingsheng Zhang
School of Life Science/Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang, 550025, China
Author    Correspondence author
Medicinal Plant Research, 2022, Vol. 12, No. 5   doi: 10.5376/mpr.2022.12.0005
Received: 03 Nov., 2022    Accepted: 11 Nov., 2022    Published: 18 Nov., 2022
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This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Tian L., Gao Y.Y., Yang N.X., Peng S.J., and Zhang M.S., 2022, Effects of plant growth regulators for seed embryos development of Cremastra appendiculata, Medicinal Plant Research, 12(5): 1-6 (doi: 10.5376/mpr.2022.12.0005)


In order to understand the process of embryo development of Rhododendron seed embryo under different plant growth regulators, the seed of Rhododendron was used as the experimental material for the rapid development and maturation of Cremastra appendiculata seeds embryo. Using Cremastra appendiculata seeds as experimental materials, the plant growth regulators naphthaleneacetate (NAA), 6-benzylaminopurine (6-BA), kinetin (KT), zeatin (ZT), beta-indolebutyric acid (IBA), Effects of 2, 4-dichlorophenoxyacetic acid (2, 4-D) on the development of azalea seed embryos. The results showed that the addition of six plant growth regulators to the medium had different effects on the development of azalea seed embryos. 2,4-D inhibited the development of embryo; adding KT and ZT, the seeds began to expand after 40 days, and the development of embryo was slow; NAA, IBA and 6-BA could accelerate the growth of embryo, and NAA had the best effect on promoting the development of embryo when it was added alone, in which NAA and 6-BA were used together and NAA: 6-BA=1 mol/L:1 mol/L, the speed of embryo development was the fastest, and the germination rate of seed reached 88.11% after 40 days. Therefore, it is necessary to fill the blank in the field of embryo development of Rhododendron so as to provide theoretical basis for the construction of rapid propagation system of Cremastra appendiculata seedlings.

Cremastra appendiculata; Seed embryo development; Plant growth regulator
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