Meirong Ye , Xiaopeng Wang , Yuli Zhou

College of Life and Health Sciences, Anhui Science and Technology University, Fengyang, 233100, China
Author    Correspondence author
Medicinal Plant Research, 2022, Vol. 12, No. 4   doi: 10.5376/mpr.2022.12.0004
Received: 03 Aug., 2022    Accepted: 09 Aug., 2022    Published: 15 Aug., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Ye M.R., Wang X.P., and Zhou Y.L., 2022, The differences and genetic relationships of 4 Dendrobium species based on high throughput GBS-SNP markers, Medicinal Plant Research, 12(4): 1-10 (doi: 10.5376/mpr.2022.12.0004)

The differences and genetic relationships of 4 Dendrobium species (Dendrobium huoshanense, Dendrobium officinale, Dendrobium moniliforme, Dendrobium Fanjingshanense) were analyzed based on high throughput SNP markers obtained by GBS, using Dendrobium catenatum as the reference genome, for providing reference for the study of these 4 Dendrobium species. The results showed that the number of clean reads per each sample in D. huoshanense, D. officinale, D. moniliforme, D. Fanjingshanense were 1 300 724, 1 286 162, 1 380 009 and 1 170 337, respectively, and the number of SNPs per each sample were 1 507 746, 893 333, 1 364 605 and 1 227 006, respectively. The genetic structure based on Admixture software revealed that the samples of D. officinalewere clustered into one branch, and the remaining Dendrobium samples were clustered into the other branch, when the best K value was 2; when k = 3, the samples of D. officinale were grouped into one cluster, some samples of D. huoshanense, all samples of D. moniliforme and D. fanjingshanense were grouped into the second cluster, and some samples of D. huoshanense were grouped into the third cluster; When k = 4, it was divided into four branches: the samples of D. officinale were grouped one branch, the samples of D. moniliforme and D. fanjingshanense were grouped one branch, the samples of D. huoshanensewere divided into two branches, which was similar to the result of an unrooted neighbor-joining phylogenetic tree (NJ tree); Furthermore, principal coordinates analyses (PCoA) revealed that 91 Dendrobium samples were classed into three cluster. All samples of D. officinale and D. huoshanense were grouped together, respectively, and the samples of D. moniliforme and D. fanjingshanense were grouped together. According to the results of this study, the genetic relation of D. officinale and the other 3 species of Dendrobium are relatively distant, and the genetic relationship of D. moniliforme and D. fanjingshanense were relatively close, 72 samples of D. huoshanense in the study were divided into two branches, the genetic relationship in one of which was relatively close to D. moniliforme and D. fanjingshanense. The study verified that the genetic relationship between different Dendrobium species or different species of plants was investigated using SNPs detected by GBS.

SNP; Dendrobium; Clean reads; NJ tree