SNP Molecular Markers Development and Genetic Diversity Analysis of Forsythia suspensa Based on SLAF-seq Technology
Hebei Medicinal Plant & Technology Innovation Center, Institute of Cash Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, 050051, China
Medicinal Plant Research, 2022, Vol. 12, No. 2 doi: 10.5376/mpr.2022.12.0002
Received: 14 Apr., 2022 Accepted: 22 Apr., 2022 Published: 09 May, 2022
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This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Jiang T., Wen C.X., Tian W., Xie X.L., Lu R.K., Wen S.Q., and Liu L.D., 2022, SNP Molecular markers development and genetic diversity analysis of Forsythia suspensa based on SLAF-seq technology, Medicinal Plant Research, 12(2): 1-10 (doi: 10.5376/mpr.2022.12.0002)
Forsythia suspensa (Thunb.) Vahl is an important medicinal plant that has great value to study in China. In this study, the 39 Forsythia suspensa materials were used specific loci amplified fragment sequencing technology (SLAF-seq) to develop SNP molecular markers. A total of 112.28 Mb reads data were obtained by sequencing. The reads data of each sample ranged from 1 324 860~5 911 565. The average sequencing quality value (Q30) and GC content of samples was 96.29% and 36.97%, respectively. Analysis of bioinformatics, there were 535 357 SLAF tags, in which 262 297 SLAF tags were polymorphic, and the average sequencing depth of the samples was 16.20 x. A total of 1 809 741 SNP molecular markers were obtained and 39 Forsythia suspensamaterials were divided into 4 groups. This study could provide theoretical basis for germplasm resource identification and genetic diversity analysis of Forsythia suspensa.
Forsythia suspensa (Thunb.) Vahl; SNP; SLAF-seq; Molecular marker