Jianfeng Cao , Ying Zhang , Peiyu Chen , Chao Wu , Yu Deng

School of Biological Sciences of Guizhou Education University, Guiyang, 550018, China
Author    Correspondence author
Medicinal Plant Research, 2021, Vol. 11, No. 4   doi: 10.5376/mpr.2021.11.0004
Received: 22 Dec., 2021    Accepted: 24 Dec., 2021    Published: 31 Dec., 2021

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cao J.F, Zhang Y., Chen P.Y., Wu C., and Deng Y., 2021, Analysis of transcriptome sequencing and related genes of flavonoid biosynthesis from Thesium chinense Turcz, Medicinal Plant Research, 11(4): 1-11 (doi: 10.5376/mpr.2021.11.0004)

In order to find the metabolic pathway of Thesium chinense Turcz flavonoids and related genes, the plants treated with or without silver nitrate (1 mmol/L) were used as test materials, transcriptome sequencing analysis was carried out by using Illumina HiSeq 2000, and analyses of gene functional annotation of unigenes and expression characteristics of key genes for biosynthesis of T. chinense Turcz flavonoids were also performed. The results showed that a total of 69 503 unigenes were obtained by transcriptome sequencing, of which 48 666 were annotated and 9 792 genes were screened by differential gene expression, with an average length of 1 226 bp. Through the metabolic pathway analysis, we found that the expression of CHS, C4H, 4CL, and HCT genes was high, and the expression of PAL, F3H, FLS and FG3 genes was relatively low among the genes closely related to the synthesis of kaempferol and flavonoids. Combined with transcriptome data, the biosynthetic pathway of flavonoid content in T. chinense Turcz was plotted. In addition, 32 008 simple sequence repeat (SSR) loci were determined in the transcriptome of T. chinense Turcz. Dinucleotide microsatellite repeating units were the most abundant in T. chinense Turcz, which accounted for 34.22%. Among the two repeating types of mononucleotide and dinucleotide, the most ascendant repeating units were A/T and AG/CT. This study provided the theoretical basis of molecular pharmacology for improving the yield, the formation of the quality and studying the pathways and related genes of biosynthesis of flavonoids of T. chinense Turcz.

Thesium chinense Turcz; Transcriptome; Flavonoids; Biosynthesis