SSR Site Information Analysis of The Transcriptome of Glehnia littoralis
1 Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China
2 The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, 210014, China
Medicinal Plant Research, 2021, Vol. 11, No. 3 doi: 10.5376/mpr.2021.11.0003
Received: 09 Dec., 2021 Accepted: 17 Dec., 2021 Published: 24 Dec., 2021
© 2021 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Li L.L., Zhou Y.F., Xu M., Li M.M., and Xu Z.L., 2021, SSR site information analysis of the transcriptome of Glehnia littoralis, Medicinal Plant Research, 11(3): 1-6 (doi:10.5376/mpr.2021.11.0003)
Glehnia littoralis, also known as Beishashen, is a medicine and food homologous plant and is one of the national second-class protected plants. The natural populations of G. littoralis are scarce. In this study, MISA (MicroSatellite) was used to search and analysis the SSRs from the transcriptome data of G. littoralis.As a result, 14 421 SSRs were identified in 78 828 unigenes, accounting for 15.43%. The numbers of SSR loci ranged from 1 to 6. The mononucleotide and dinucleotide are the most frequent types, accounting for 39.98% and 40.98%, respectively. The repeat motifs of SSR ranged from 5 to 24, of which 6 and 10 were the most, with 3 102 and 2 672 respectively, accounting for 21.51% and 18.53%. A total of 2 894 pairs of primers were designed by Primer 6.0 software, of which, 20 pairs of primers were randomly selected for amplification and validation, and 12 pairs of primers successfully amplified. The above results showed that the SSR loci in the transcriptome of G. littoralis provided technical basis for the construction of genetic map, analysis of genetic diversity, species identification and breeding of G. littoralis.
Glehnia littoralis; Transcriptome; SSR site