To evaluate the invivo antioxidant activities of methanol (MEAL) and aqueous extracts (AEAL) of aerial parts of Aerva lanata Linn Juss in streptozotocin induced oxidative stress rats which are used in the folklore system for the treatment of diabetes mellitus in India. Rats were divided into seven groups of six rats (n=6) each. Group 1 served as vehicle control using normal saline (0.9 % w/v NaCl), Group 2 served as diabetic control, Group 3 served as standard treated with 0.5 mg/kg of glibenclamide, Group 4 and 5 was treated with MEAL 200 and 400 mg/ kg, p.o., Group 6 and 7 was treated with AEAL 200 and 400 mg/kg, p.o. Phenolic content, antioxidant enzymes, liver glycogen and lipid peroxidation were evaluated and compared with diabetic control. MEAL (400 mg/kg) showed significant increase in the protein, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), reduced glutathione (GSH), hexokinase and liver glycogen levels (p<0.01) than AEAL when compared with diabetic control. The MEAL at the dose of 400 mg/kg showed significant decrease in thiobarbituric acid reactive substance (TBARS) levels, Glucose 6-phosphatase (G6-pase), Fructose 1,6 biphosphatase (FD-pase). Thus, these results scientifically that confirm that MEAL at the higher dose may effectively normalize the various disorders caused by free radicals and other chemical substances due to the presence of secondary metabolites that exert antioxidant activity.