Tuğçe DEM?R¹ , Mehmet Özgün Ã–ZEN^1,2 , E. Esin HAMEÅž-KOCABAÅž¹

1. Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey 2. Department of Bioengineering, Faculty of Engineering, Gümüşhane University 29100 Gümüşhane, Turkey
Author    Correspondence author
Medicinal Plant Research, 2014, Vol. 4, No. 4   doi: 10.5376/mpr.2014.04.0004
Received: 17 Feb., 2014    Accepted: 25 Feb., 2014    Published: 28 Feb., 2014

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Physalis peruviana has widely used in folk medicine as a medicinal herb for treating diseases since ancient times. It is a pharmacologically valuable natural product due to the presence of biologically active compounds such as phytosterols, vitamins, essential minerals, withanolides and physalins. In this study, we aimed to observe the antioxidant capacity and cytotoxic activity of edible parts of P. peruviana on different cancer cell lines [human colon adenocarcinoma cell line (HT-29), human prostate adenocarcinoma cell line (LNCap), human hepatoma cell line (Hep3B), human breast adenocarcinoma cell line (MCF-7), human neuroblastoma cell line (SH-SY5Y), human osteosarcoma cell line (SaOS-2)] and a non-cancerous kidney epithelial cells from African green monkey (Vero) cell line. Antioxidant activity of the fruit extracts was determined with DPPH (1,1-diphenyl-2-picrylhidrazil) radical scavenging method and cytotoxic effect was observed by using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide) assay. For antioxidant capacity of the crude extract, IC₅₀ value was calculated as 0.43±0.003 mg/ml. IC₅₀values of cytotoxicity for HT-29, Hep3B, SaOS-2 and SH-SY5Ycell lines for 48 h were determined as 40.79, 24.92, 15.44 and 44.24 µg/ml, respectively. Fruit extract of P. peruviana had no cytotoxic effect on MCF-7, LNCap and Vero cell lines.

Antioxidant activit; Cytotoxic activity; DPPH; Physalis peruviana