Research Article

Genetic Diversity Analysis and DNA Fingerprinting Construction with SSR Markers for Tea Germplasm Resources from Duyun  

Yuxian Yao , Mingze Zhang , Liping Liu , Shijun Chen , Jing Li
College of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun, 558000, China
Author    Correspondence author
Journal of Tea Science Research, 2023, Vol. 13, No. 1   doi: 10.5376/jtsr.2023.13.0001
Received: 03 Jul., 2023    Accepted: 12 Jul., 2023    Published: 25 Jul., 2023
© 2023 BioPublisher Publishing Platform
Preferred citation for this article:

Yao Y.X., Zhang M.Z., Liu L.P., Chen S.J., and Li J., 2023, Genetic diversity analysis and DNA fingerprinting construction with SSR markers for tea germplasm resources from Duyun, Journal of Tea Science Research, 13(1): 1-9 (doi: 10.5376/jtsr.2023.13.0001)

Abstract

In order to understand the genetic background and genetic differences of different tea germplasm sources, the genetic diversity and molecular fingerprinting of 11 tea germplasms collected from the origin of Duyun Maojian were analyzed by using SSR markers. The results showed that a total of 138 observed alleles and 89.773 2 effective alleles were found using 15 SSR markers with a mean of 9.2 and 5.984 9 per locus. The average values of PIC and Shannon’s information index were 0.810 4 and 1.975 8, respectively, indicating that there was higher level of diversity in the tested germplasms. The Nei’s genetic distance between 11 tea germplasms varied from 0.667 7 to 3.736 5, indicating that the genetic variation was large among them. When the threshold of coefficient was 0.30, 11 tea germplasms were clustered into three composite categories. 10-digit fingerprint database code of each tea germplasm was obtained based on five core SSR markers, DNA fingerprinting constructed with fingerprint database codes can quickly identify these germplasm resources.

Keywords
Tea (Camellia sinensis); Germplasm resources; SSR; Molecular fingerprinting; Original habitat
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