Analysis of CYP Supergene Family in Citrus clementina and the Characterization of a CYP gene encoding Flavonoid 3'-hydroxylase
Published:31 Jan.2023 Source:Hortic Res
Cytochrome P450 monooxygenase (CYP) is the largest enzyme family in plants. It is widely involved in many monooxygenase reactions such as hydroxylation, epoxidation and demethylation, and plays a vital role in plant growth and development and defense. Citrus clementina is a major fruit in the world. Its rich primary and secondary metabolites (such as terpenes and flavonoids) directly or indirectly affect the quality of citrus fruit. The CYP superfamily is the key enzyme for the biosynthesis of these metabolites. However, the information about the CYP superfamily in Citrus clementina is very limited.
Recently, Horticulture Research launched (Advance Access) a review entitled "Genome-wide analysis of cytochrome P450 genes in Citrus clementina and characterization of a CYP gene encoding flavonoid 3'-hydroxylase ". In this study, the CYP superfamily of Citrus clementina was first identified and analyzed at the genome-wide level based on the CYP enzyme system nomenclature. A total of 301 CYP genes were identified and divided into 10 clans and 49 families. It is noteworthy that researchers have found one or more photoresponsive cis-elements in the promoters of most citrus CYP members; Moreover, transcriptome data confirmed that some CYP members related to flavonoids synthesis were induced by UV-B radiation.
This information will provide important data support for understanding the function and classification of CYP superfamily in Citrus clementina, and also provide important reference value for the functional characterization of members of this family in the future.