Analysis of the Mechanism of SmMYB36-SmERF6/SmERF115 Regulating the Biosynthesis of Tanshinones and Phenolic Acids in Salvia miltiorrhiza Hairy Roots
Published:05 Dec.2022    Source: Hortic Res

Dānshēn (Salvia miltiorrhiza Bge.), a traditional Chinese medicine, is the dried root and stem of Salvia miltiorrhiza, a plant of Salvia genus in the family of Labiatae, which contains rich tanshinones and phenolic acids. Tanshinone and phenolic acids are the most important active substances of Salvia miltiorrhiza, and the insight into their transcriptional regulatory mechanisms is an essential process to increase their content in vivo. SmMYB36 has been found to have important regulatory functions in the synthesis of tanshinone and phenolic acid, paradoxically, its mechanism of action in S. miltiorrhiza is not clear.

Recently, the mechanism that SmMYB36-SmERF6/SmERF115 regulates the biosynthesis of tanshinone and phenolic acids has been analyzed by Northwest A&F University. Here, researchers demonstrated that SmMYB36 functions as a promoter of tanshinones accumulation and a suppressor of phenolic acids through the generation of SmMYB36 overexpressed and chimeric SmMYB36-SRDX (EAR repressive domain) repressor hairy roots in combination with transcriptomic-metabolomic analysis.

SmMYB36 has functional pleiotropy in regulating the synthesis of tanshinone and phenolic acids. A transcription factor acts in a variety of ways and plays a variety of regulatory roles, which lays a foundation for a comprehensive understanding of the regulatory mechanism of active components of S. miltiorrhiza, and provides a useful tool for improving the quality of S. miltiorrhiza and accurately regulating active components by genetic engineering in the future.