Xinchao Cao

Caonong Herbal Health Food Business Department, Lin'an District, Hangzhou, 311300, Zhejiang, China
Author    Correspondence author
Medicinal Plant Research, 2026, Vol. 16, No. 2   
Received: 27 Feb., 2026    Accepted: 04 Apr., 2026    Published: 16 Apr., 2026

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Dendrobium officinale is an important medicinal orchid plant in China with both medicinal and food uses. It has high medicinal value, health-care value, and industrial development potential. However, its wild resources are limited, and the efficiency of conventional propagation is relatively low, making it difficult to meet the demand for large-scale and standardized seedling production. Tissue culture rapid propagation provides an important technical pathway for the rapid multiplication of high-quality D. officinale seedlings and the conservation of germplasm resources. This study explored technologies for tissue culture rapid propagation and improvement of transplant survival rate in D. officinale, with emphasis on key links including explant selection, establishment of an aseptic culture system, optimization of culture media and nutritional conditions, protocorm induction and subculture proliferation, multiple shoot differentiation, rooting and strong seedling cultivation, seedling grading before bottle removal, hardening-off acclimatization, and transplant management. The results indicate that the rapid propagation efficiency of D. officinale is jointly affected by explant type, basal medium, plant growth regulators, organic additives, activated carbon, and subculture cycle. Meanwhile, transplant survival rate is closely related to root quality, seedling vigor, acclimatization intensity, substrate formulation, temperature and humidity management, and beneficial microbial interactions. By constructing a continuous technical chain of “induction-proliferation-rooting-strong seedling cultivation-hardening-off-transplanting,” seedling uniformity, transplant adaptability, and production stability can be effectively improved. In the future, further efforts should be made to strengthen research on the standardization of D. officinale tissue culture systems, seedling quality evaluation, intelligent management, and microbial synergistic regulation, thereby providing technical support for the industrialized, standardized, and sustainable development of D. officinale.

Dendrobium officinale; Tissue culture rapid propagation; Protocorm; Strong seedling cultivation; Transplant survival rate