Huixian Li , Jianhui Li

Institute of Life Science, Jiyang College of Zhejiang A&F University, Zhuji, 311800, Zhejiang, China
Author    Correspondence author
Medicinal Plant Research, 2025, Vol. 15, No. 4   doi: 10.5376/mpr.2025.15.0016
Received: 10 May, 2025    Accepted: 15 Jun., 2025    Published: 02 Jul., 2025

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Li H.X., and Li J.H., 2025, Physiological study on root adaptation and recovery of tissue-cultured Anoectochilus roxburghii seedlings after transplanting, Medicinal Plant Research, 15(4): 151-160 (doi: 10.5376/mpr.2025.15.0016)

 

Anoectochilus roxburghii, a rare medicinal plant, has attracted widespread attention for its scarce resources and high cultivation difficulty. Tissue culture rapid propagation, has become the main seedling raising method, but the adaptation and recovery of the root system after transplantation remain a bottleneck. This study summarizes several regulatory mechanisms: the proportional relationship of hormones (IAA, CK, stellolactone), the accumulation of osmotic regulatory substances (proline, soluble sugar), and the activation of antioxidant systems (SOD, CAT, POD), which play an important role in alleviating transplant shock. Meanwhile, the upregulation of energy metabolism, the accumulation of secondary metabolites, and the absorption of mineral elements have been proven to be the key supports for the rejuvenation of root systems. Studies show that, a reasonable hormone ratio can shorten the recovery period of the root system. The accumulation of osmotic regulatory substances, and the enhancement of antioxidant enzyme activity, play a prominent role in the early recovery stage. In the later stage, light quality regulation and substrate improvement have shown obvious effects on the improvement of root function, and the overall adaptation of the plant. This study provides new ideas for the efficient cultivation and management of A. roxburghii, and also offers theoretical basis and guidance for its industrial promotion and resource protection.

Anoectochilus roxburghii; Transplant shock; Root recovery; Osmotic regulation; Growth hormone