Chuchu Liu¹ , Yuhong Huang²

1 Institute of Life Science, Jiyang College of Zhejiang A&F University, Zhuji, 311800, Zhejiang, China
2 Traditional Chinese Medicine Research Center, Cuixi Academy of Biotechnology, Zhuji, 311800, Zhejiang, China
Author    Correspondence author
Medicinal Plant Research, 2025, Vol. 15, No. 3   doi: 10.5376/mpr.2025.15.0013
Received: 10 Apr., 2025    Accepted: 28 May, 2025    Published: 07 Jun., 2025

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Liu C.C., and Huang Y.H., 2025, Effects of growth regulators on root development and antioxidant capacity of Anoectochilus roxburghii tissue culture seedlings, Medicinal Plant Research, 15(3): 119-128 (doi: 10.5376/mpr.2025.15.0013)

 

The wild resources of Anoectochilus roxburghii are becoming increasingly scarce, and breakthroughs are still needed in tissue culture rapid propagation in terms of root growth, and stress resistance. This study sorted out the effects of auxin (IBA, NAA), cytokinin (6-BA), novel regulatory factors, and light quality on root development and physiological functions. The results showed that, the rooting rate of MS+1.0 mg/L IBA+1.0 mg/L NAA was as high as 92%, and the average number of roots was 2.62. 1.0 mg/L 6-BA+0.05 mg/L NAA, can promote bud cluster formation. In proliferation culture, B5 medium and red light are more beneficial. The 1:1 combination of red and blue light can simultaneously optimize growth and polyamine metabolism, while blue light enhances the activities of SOD, POD and CAT. When the transplanting substrate is peat to vermiculite in a ratio of 3:1, the survival rate reaches 80%. Regulators can also promote the accumulation of flavonoids and polysaccharides, and form a synergistic effect with exogenous polyamines and strigolactone, enhancing antioxidant capacity and drought resistance. This study provides a technical path for the sustainable cultivation and improvement of medicinal quality of A. roxburghii, and also offers a reference for the rapid propagation and resource conservation of related medicinal plants.

Anoectochilus roxburghii; Growth regulator; Tissue culture; Growth hormone; Antioxidant capacity; Medicinal quality