Case Study
Effects of Different Transplanting Substrates on the Survival Rate and Growth Quality of Anoectochilus roxburghii Tissue Culture Seedlings 
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Medicinal Plant Research, 2025, Vol. 15, No. 2 doi: 10.5376/mpr.2025.15.0009
Received: 12 Feb., 2025 Accepted: 21 Mar., 2025 Published: 18 Apr., 2025
Li C.Y., and Zhou J.Y., 2025, Effects of different transplanting substrates on the survival rate and growth quality of Anoectochilus roxburghii tissue culture seedlings, Medicinal Plant Research, 15(2): 80-87 (doi: 10.5376/mpr.2025.15.0009)
Anoectochilus roxburghii is a precious and rare medicinal plant with extensive uses in traditional Chinese medicine due to the richness of bioactive compounds and unusual pharmacological activities. Tissue culture seedlings are a primary tool for A. roxburghii cultivation, but their survival ratio and growth quality during the transplanting phase are determined by a series of factors among which the transplanting substrate selection is particularly crucial. With the technology of cultivating research being a key component of the discipline, the effects of different transplant substrates on plant growth have been of special interest. In this review, the biological properties of A. roxburghii and the progress in its bioactive compounds research are detailed systemically. It also explains the main mechanisms in which transplanting substrates impact plant growth, especially the interactions among substrate physical and chemical properties (e.g., water holding capacity, aeration, and nutrient supply) and seedling survival rate and growth quality. Moreover, it indicates successful experience and technical issues in substrate choice both at home and abroad and elaborates on how to optimize and manage substrates for promoting the industrial development of A. roxburghii. This review provides scientific citations for study and use of transplanting media of A. roxburghii, driving the development of successful cultivation technologies.
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. Anoectochilus roxburghii
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