Xiazhen  Huang¹ , Yufen  Wang²

1 Tropical Medicinal Plant Research Center, Hainan Institute of Tropical Agricultural Resources, Sanya, 572025, Hainan, China
2 Traditional Chinese Medicine Research Center, Cuixi Academy of Biotechnology, Zhuji, 311800, Zhejiang, China
Author    Correspondence author
Medicinal Plant Research, 2025, Vol. 15, No. 2   doi: 10.5376/mpr.2025.15.0007
Received: 02 Jan., 2025    Accepted: 08 Feb., 2025    Published: 15 Mar., 2025

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Huang X.Z., and Wang Y.F., 2025, Application of heat treatment and tissue culture combined detoxification technology in the healthy seedling propagation of Lindera aggregata, Medicinal Plant Research, 15(2): 62-70 (doi: 10.5376/mpr.2025.15.0007)

This study discusses the application of integrated heat treatment and tissue culture technology for detoxifying Lindera aggregata seedlings, determining its efficiency in detoxification and healthy seedling development, and its usability and value for further implication. The study has revealed that tissue culture technology readily increases the rate of propagation of Lindera aggregata seedlings using shoot tip culture technology with optimal media conditions promoting shoot proliferation and differentiation. Integrated technology enhances substantially detoxification efficacy along with genetic stability and resistance characteristics of Lindera aggregata seedlings. Thermal treatment suppresses viral replication at elevated temperatures, whereas tissue culture utilizes sterile environment and shoot tip isolation to remove viruses. Combined, these treatments attain detoxification efficacy of over 90%. Detoxified seedlings show better growth characteristics and increased quantities of medicinal constituents, including essential oils and polysaccharides, than control seedlings. The detoxified seedlings also have enhanced growth vigor and stress resistance as well as remarkable active ingredient content promotion. The integration of heat treatment and tissue culture technology in the cultivation of healthy Lindera aggregata seedlings not only increases detoxification efficiency and seedling multiplication but also reduces the risk of disease transmission, rationalizes cultivation management, and guarantees the quality of Lindera aggregata products. This research provides theoretical foundations and technical backstopping for Lindera aggregata germplasm resource preservation and utilization in industry.

Lindera aggregata; Detoxification technology; Heat treatment; Tissue culture; Healthy seedlings