Baofu Huang , Guangman Xu

Traditional Chinese Medicine Research Center, Cuixi Academy of Biotechnology, Zhuji, 311800, Zhejiang, China
Author    Correspondence author
Medicinal Plant Research, 2025, Vol. 15, No. 2   doi: 10.5376/mpr.2025.15.0006
Received: 15 Dec., 2024    Accepted: 20 Jan., 2025    Published: 10 Mar., 2025

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Huang B.F., and Xu G.M., 2025, Research insight into enhancing triterpenoid content through genetic modification in Ganoderma lucidum, Medicinal Plant Research, 15(2): 51-61 (doi: 10.5376/mpr.2025.15.0006)

Ganoderma lucidum has been widely studied and applied due to its high medicinal value. Its triterpenoid compounds are one of the main active ingredients. But, it is not easy to stably increase the triterpenoid content using traditional cultivation methods, which is a common bottleneck in the industrialization process. In order to solve this problem, many studies have begun to focus on gene-level regulation. For example, using new gene editing technologies such as CRISPR/Cas9, the key genes responsible for triterpenoid synthesis in G. lucidum can be accurately regulated. At the same time, transcription factors such as GlbHLH5 have also been shown to promote triterpenoid synthesis, indicating that the content can be increased from multiple links. Overall, this type of genetic improvement method has indeed opened up a new situation, which is expected to increase triterpenoid production and provide a more stable source of raw materials for the development of functional foods and medicinal products of G. lucidum.

Ganoderma lucidum; Triterpenoids; Gene editing; Transcription factors; Functional foods; Medicinal value