Genetic Diversity Analysis of Youxi Bitter Tea Resources Based on ISSR Molecular Markers
1 College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China
2 Collaborative Innovation Center of Modern Agriculture Industrial Park of Anxi County, Quanzhou, 362400, China
3 Fujian Provincial Technology Development Base of Tea Industry, Fuzhou, 350002, China
4 Anxi College of Tea Science, Fujian Agriculture and Forestry University, Quanzhou, 362400, China
5 Yuxi County Tangchuan Township High Mountain Tea Professional Cooperative, Sanming, 365100, China
6 Science and Innovation Center, Guangdong Degaoxin Food Processing Co., Ltd., Qingyuan, 513031, China
Journal of Tea Science Research, 2020, Vol. 10, No. 3 doi: 10.5376/jtsr.2020.10.0003
Received: 04 Nov., 2020 Accepted: 05 Nov., 2020 Published: 13 Nov., 2020
© 2020 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Wei S.S., Peng J., Chen Z.D., Sun W.J., Lin L., and Wu R.Q., 2020, Genetic diversity analysis of Youxi bitter tea resources based on ISSR molecular markers, Journal of Tea Science Research, 10(3): 1-11 (doi: 10.5376/jtsr.2020.10.0003)
In this study, we analyzed the genetic diversity by using ISSR markers in four populations with 37 DNA samples of Youxi Kucha. In total, 66 alleles were amplified using 8 ISSR primers. A total of 66 bands were detected, of which 63 bands were polymorphic with a polymorphic proportion of 95.24 %. At the population level, the average PPL of the four populations of Youxi Kucha is 76.19 %, and the average values of Nei's gene diversity index (H) and Shannon diversity index (I) are 0.204 5 and 0.367 7 respectively. At the species levelm the H and I are 0.305 1 and 0.465 0 respectively respectively. Youxi Kucha maintained a relatively high genetic variability at the population level and species level bothly. The genetic differentiation within the population was significantly higher than that between populations，but the genetic differentiation between the populations has also reached higher level (Φst = 0.17> 0.15), the differentiation is extremely significant (P <0.01). A mantel test indicated there was no significant relationship between genetic distance and geographic distance among the populations studied. Correlation analysis shows that the diversity index has little relationship with altitude. This study carried out the molecular identification of the germplasm resources of Youxi bitter tea accurately, which can provide a theoretical basis for the protection of Youxi bitter tea and the breeding of improved tea varieties.
Youxi Kucha; ISSR; Genetic diversity; Genetic distance; Genetic differentiation