Analysis of DNA Barcoding Suitable for Tea Tree Field Genebank
Wuyi University, Wuyishan, 354300
Journal of Tea Science Research, 2020, Vol. 10, No. 2 doi: 10.5376/jtsr.2020.10.0002
Received: 03 Sep., 2020 Accepted: 08 Sep., 2020 Published: 25 Sep., 2020
© 2020 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Li Y.Y., Huang W., Lin J.J., and Nie C.P., 2020, Analysis of DNA barcoding suitable for tea tree field genebank, Journal of Tea Science Research, 10(2): 1-7 (doi: 10.5376/jtsr.2020.10.0002)
With the development of research for DNA barcoding, its application has attracted more and more attention. In this study, 100 tea tree samples were selected as subjects, the partial sequences of chloroplast matK and rbcL genes were used to investigate the molecular barcodes suitable for tea tree The results showed that the rbcL sequences of 100 tea samples were identical, the matK sequences were different, the genetic distance ranged from 0.000 to 0.032, the sequences could be divided into 14 haplotypes, Hd and Pi were 0.604 and 0.23×10-2, at the same time, the construction analysis of the sequence is carried out. The results showed that the matKsequence could be used in the development and utilization of DNA barcoding of tea tree field genebank.
Tea tree; DNA barcoding; Field genebank
Journal of Tea Science Research
• Volume 10