Locus R-D1 Conferring Red-Grain-Color in Synthetic Derivative Wheat Chuanmai 42 Mapped with SSR Markers
1. Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, P.R. China
2. Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, P.R. China
3. Institute of Biodiversity Science, Fudan University, Shanghai, 200433, P.R. China
Molecular Plant Breeding, 2010, Vol. 1, No. 3 doi: 10.5376/mpb.2010.01.0003
Received: 06 Apr., 2010 Accepted: 29 Jun., 2010 Published: 28 Sep., 2010
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Preferred citation for this article:
Li et al., 2010, Locus R-D1 conferring red-grain-color in synthetic derivative wheat chuanmai 42 mapped with SSR markers, Molecular Plant Breeding Vol.1 No.3 (doi:10.5376/mpb.2010.01.0003)
Synthetic hexaploid wheat (SHW) (Triticum durum×Aegilops tauschii) holds a large number of unfavorable traits, of which, red grain color was important to limit the wheat breeder using SHWs for common wheat improvement. The objectives of this study were to identify and map the red grain color gene derived from SHW in a Chinese wheat variety Chuanmai 42 by SSR molecular markers. 1015 individuals in F2 population derived from the cross of Chuanmai 42 with a red grain color and ChuanW565 (CW565) with white grain color were used for tagging the gene of red grain color. A total of 40 SSR markers on the chromosome 3D were employed to detect the polymorphism of between the parents, Chuanmai 42 and CW565. 241 recessive individuals in F2 population derived from the cross (Chuanmai 42×CW565) were employed for allelic test of the red-grain gene by eight polymorphic markers. The results indicated that the red-grain color gene of Chuanmai 42 derived from the D genome donor of synthetic hexaploid wheat and controlled by a single dominant gene R-D1 on the long arm chromosome 3D within an interval of 4.6 flanked by SSR markers Xgwm3 and Xgwm314, and genetic distance were 2.1 cM, 2.5 cM, respectively. Those two SSR markers can be used in marker-assisted selection for developing new white grain wheat variety based on the progenies of SHWs or Chuanmai 42 crossed with white grain color wheat in wheat breeding program.
Synthetic hexaploid wheat; Red-grain color; Aegilops tauschii; Chuanmai 42; SSR