Arvind Kumar Verma , K.V. Prasad , T.J. Anakiram , S. Kumar

Division of Floriculture and Landscaping, Indian Agricultural Research Institute, New Delhi, 110 012, India
Author    Correspondence author
International Journal of Horticulture, 2012, Vol. 2, No. 3   doi: 10.5376/ijh.2012.02.0003
Received: 10 Dec., 2012    Accepted: 17 Dec., 2012    Published: 26 Dec., 2012

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Arvind K.V.,  Prasad  K.V., Anakiram T.J., and Kumar S., 2012, Standardization of Protocol for Pre-treatment, Surface Sterilization, Regeneration, Elongation and Acclimatization of Chrysanthemum morifolium Ramat, International Journal of Horticulture, 2(3): 7-12 (doi: 10.5376/ijh.2012.02.0003)

In the present study we have developed proficient in vitro regeneration protocol from the ray florets of chrysanthemum cv. Thai Chen Queen to isolate the novel mutants. The maximum survival percentage (89.44) of cultures was found when the ray florets were per-treated with mancozeb-45 (0.1%)+carbendazim (0.1%)+8-HQC (200 mg/L) for 3 hours. Culture establishment was found best (91.04%) when the ray florets surface sterilized with HgCl₂ (0.1%) for duration of four minutes. Murashige and Skoog (MS) media supplemented with 4 mg/L BAP and 0.1 mg/L NAA was found to the best for regeneration percentage (93.33), number of microshoots per explants (5.67) and minimum days required for shoot regeneration (30.67). The regenerated plantlets were transferred on the MS media supplemented with GA3 0.50 mg/L was found to the best for shoot elongation after 30 days of elongation. The successful acclimatization of elongated plantlets was achieved by transferring them into glass jar with polypropylene cap filled with peat+soilrite (1:1). The hardening of plantlets was completed after 25 days of acclimatization and then plants were transferred to greenhouse for flowering.

Chrysanthemum; Chrysanthemum morifolium; Growth regulators; In vitro culture; Shoot regeneration; Acclimatization