Immunohistochemical Research of the Expression of Nile Tilapia (Oreochromis niloticus) NCCRP-1 in Various Tissues
1 Fisheries College of Guangdong Ocean University, Zhanjiang, 524088, China
2 Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemilogy for Aquatic Economic Animals, Zhanjiang, 524088, China
3 Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, 524088, China
Genomics and Applied Biology, 2018, Vol. 9, No. 9 doi: 10.5376/gab.2018.09.0009
Received: 16 Oct., 2018 Accepted: 05 Nov., 2018 Published: 29 Dec., 2018
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This article was first published in Genomics and Applied Biology (2018, 06: 2356-2360) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Zheng Q., Huang Y., Cai J., Yuan M., Tang J.F., Lu Y.S., Wu Z.H., and Jian J.C., 2018, Immunohistochemical research of the expression of Nile tilapia (Oreochromis niloticus) NCCRP-1 in various tissues, Genomics and Applied Biology, 9(9): 56-61 (doi: 10.5376/gab.2018.09.0009)
It is well known that non-specific cytotoxic cell is a kind of lymphocyte in teleosts, which plays an important role in nonspecific immunity by up-regulating inflammation response and releasing cytokines. NCCs are equivalents of NK cells in mammals. NCC in fishes distributes in tissues and organs with spontaneous cytotoxicity which can kill various target cells such as allogenic or heterogeneous tumor cells, parasitic protozoan and cells induced by pathogens. NCCRP-1 is a landmark receptor on NCCs. This study sectioned brain, head kidney, liver and spleen of Nile Tilapia (Oreochromis niloticus) and stained NCCRP-1 as well. The study obtained the distribution of NCCs in tissues via immunohistochemical analysis. Furthermore, we also isolated and obtained NCC of head kidney through Percoll density gradient centrifugation, stained by immunohistochemistry and tagged with FITC. The distribution was the same as the staining result in tissues. This study observed the distribution characteristics of NCC in tissues more clearly than qPCR technique, which might lay the foundation for further studies on the function of NCC.
Nile tilapia (Oreochromis niloticus); NCCRP-1; Immunohistochemistry